Two palmitylated cysteine residues of the severe acute respiratory syndrome coronavirus spike (S) protein are critical for S incorporation into virus-like particles, but not for M-S co-localization.
Identifieur interne : 001C30 ( Main/Exploration ); précédent : 001C29; suivant : 001C31Two palmitylated cysteine residues of the severe acute respiratory syndrome coronavirus spike (S) protein are critical for S incorporation into virus-like particles, but not for M-S co-localization.
Auteurs : Makoto Ujike [Japon] ; Cheng Huang [États-Unis] ; Kazuya Shirato [Japon] ; Shutoku Matsuyama [Japon] ; Shinji Makino [États-Unis] ; Fumihiro Taguchi [Japon]Source :
- The Journal of general virology [ 1465-2099 ] ; 2012.
Descripteurs français
- KwdFr :
- Acide palmitique (métabolisme), Animaux, Assemblage viral (physiologie), Cellules COS, Cystéine (métabolisme), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (métabolisme), Protéines de l'enveloppe virale (métabolisme), Protéines de la matrice virale (métabolisme), Souris, Syndrome respiratoire aigu sévère (virologie), Virion (métabolisme), Virion (physiologie), Virus du SRAS (métabolisme), Virus du SRAS (physiologie).
- MESH :
- métabolisme : Acide palmitique, Cystéine, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines de la matrice virale, Virion, Virus du SRAS.
- physiologie : Assemblage viral, Virion, Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Cellules COS, Glycoprotéine de spicule des coronavirus, Souris.
English descriptors
- KwdEn :
- Animals, COS Cells, Chlorocebus aethiops, Cysteine (metabolism), Membrane Glycoproteins (metabolism), Mice, Palmitic Acid (metabolism), SARS Virus (metabolism), SARS Virus (physiology), Severe Acute Respiratory Syndrome (virology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (metabolism), Viral Matrix Proteins (metabolism), Virion (metabolism), Virion (physiology), Virus Assembly (physiology).
- MESH :
- chemical , metabolism : Cysteine, Membrane Glycoproteins, Palmitic Acid, Viral Envelope Proteins, Viral Matrix Proteins.
- metabolism : SARS Virus, Virion.
- physiology : SARS Virus, Virion, Virus Assembly.
- virology : Severe Acute Respiratory Syndrome.
- Animals, COS Cells, Chlorocebus aethiops, Mice, Spike Glycoprotein, Coronavirus.
Abstract
The endodomain of several coronavirus (CoV) spike (S) proteins contains palmitylated cysteine residues and enables co-localization and interaction with the CoV membrane (M) protein. Depalmitylation of mouse hepatitis virus S proteins abolished this interaction, resulting in the failure of S incorporation into virions. In contrast, an immunofluorescence assay (IFA) showed that depalmitylated severe acute respiratory syndrome coronavirus (SCoV) S proteins still co-localized with the M protein in the budding site. Here, we determined the ability of depalmitylated SCoV S mutants to incorporate S into virus-like particles (VLPs). IFA confirmed that all SCoV S mutants co-localized with the M protein intracellularly. However, the mutants lacking two cysteine residues (C(1234/1235)) failed to incorporate S into VLPs. This indicated that these palmitylated cysteines are essential for S incorporation, but are not involved in S co-localization mediated by the M protein. Our findings suggest that M-S co-localization and S incorporation occur independently of one another in SCoV virion assembly.
DOI: 10.1099/vir.0.038091-0
PubMed: 22238235
Affiliations:
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xml:lang="fr">Japon</country><wicri:regionArea>Laboratory of Virology and Viral Infections, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Huang, Cheng" sort="Huang, Cheng" uniqKey="Huang C" first="Cheng" last="Huang">Cheng Huang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555-1019, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555-1019</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Shirato, Kazuya" sort="Shirato, Kazuya" uniqKey="Shirato K" first="Kazuya" last="Shirato">Kazuya Shirato</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology III, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo 208-0011, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology III, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo 208-0011</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Matsuyama, Shutoku" sort="Matsuyama, Shutoku" uniqKey="Matsuyama S" first="Shutoku" last="Matsuyama">Shutoku Matsuyama</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology III, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo 208-0011, Japan.</nlm:affiliation><country 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Cells</term><term>Chlorocebus aethiops</term><term>Cysteine (metabolism)</term><term>Membrane Glycoproteins (metabolism)</term><term>Mice</term><term>Palmitic Acid (metabolism)</term><term>SARS Virus (metabolism)</term><term>SARS Virus (physiology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (metabolism)</term><term>Viral Matrix Proteins (metabolism)</term><term>Virion (metabolism)</term><term>Virion (physiology)</term><term>Virus Assembly (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide palmitique (métabolisme)</term><term>Animaux</term><term>Assemblage viral (physiologie)</term><term>Cellules COS</term><term>Cystéine (métabolisme)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Protéines de la matrice virale (métabolisme)</term><term>Souris</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Virion (métabolisme)</term><term>Virion (physiologie)</term><term>Virus du SRAS (métabolisme)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cysteine</term><term>Membrane Glycoproteins</term><term>Palmitic Acid</term><term>Viral Envelope Proteins</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term><term>Virion</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acide palmitique</term><term>Cystéine</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines de la matrice virale</term><term>Virion</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Assemblage viral</term><term>Virion</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term><term>Virion</term><term>Virus Assembly</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>COS Cells</term><term>Chlorocebus aethiops</term><term>Mice</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules COS</term><term>Glycoprotéine de spicule des coronavirus</term><term>Souris</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The endodomain of several coronavirus (CoV) spike (S) proteins contains palmitylated cysteine residues and enables co-localization and interaction with the CoV membrane (M) protein. Depalmitylation of mouse hepatitis virus S proteins abolished this interaction, resulting in the failure of S incorporation into virions. In contrast, an immunofluorescence assay (IFA) showed that depalmitylated severe acute respiratory syndrome coronavirus (SCoV) S proteins still co-localized with the M protein in the budding site. Here, we determined the ability of depalmitylated SCoV S mutants to incorporate S into virus-like particles (VLPs). IFA confirmed that all SCoV S mutants co-localized with the M protein intracellularly. However, the mutants lacking two cysteine residues (C(1234/1235)) failed to incorporate S into VLPs. This indicated that these palmitylated cysteines are essential for S incorporation, but are not involved in S co-localization mediated by the M protein. Our findings suggest that M-S co-localization and S incorporation occur independently of one another in SCoV virion assembly.</div></front></TEI><affiliations><list><country><li>Japon</li><li>États-Unis</li></country><region><li>Région de Kantō</li><li>Texas</li></region><settlement><li>Tokyo</li></settlement></list><tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Ujike, Makoto" sort="Ujike, Makoto" uniqKey="Ujike M" first="Makoto" last="Ujike">Makoto Ujike</name></region><name sortKey="Matsuyama, Shutoku" sort="Matsuyama, Shutoku" uniqKey="Matsuyama S" first="Shutoku" last="Matsuyama">Shutoku Matsuyama</name><name sortKey="Shirato, Kazuya" sort="Shirato, Kazuya" uniqKey="Shirato K" first="Kazuya" last="Shirato">Kazuya Shirato</name><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name></country><country name="États-Unis"><region name="Texas"><name sortKey="Huang, Cheng" sort="Huang, Cheng" uniqKey="Huang C" first="Cheng" last="Huang">Cheng Huang</name></region><name sortKey="Makino, Shinji" sort="Makino, Shinji" uniqKey="Makino S" first="Shinji" last="Makino">Shinji Makino</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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